Canine Mat Test Leptospirosis
Ideally a combination of serology and organism detection should be used for diagnosis of canine leptospirosis.
Canine mat test leptospirosis. Results in minutes support treatment recommendations and provide clients with answers about this challenging contagious disease. The bacteria infiltrate a dog s body by burrowing into their skin. Canine leptospirosis can be a serious life threatening disease. However if the patient has been vaccinated mat testing is useless.
Antibody reactivity may vary depending upon the dog and frequency of vaccination. Titers against serovar autumnalis are available in our expanded panel test code 95066 or as an add on test code 95054 by request. The mat is considered the gold standard serologic test to confirm leptospirosis. Laboratory testing is a critical component to aiding in the clinical diagnosis of this challenging disease.
2 4 for this test the patient s serum is sent to a referral laboratory where it is combined with live leptospires. Leptospirosis in dogs is a bacterial infection that spreads through the bloodstream. Infection can be diagnosed with either test but each has weaknesses and in some situations both tests may be needed to reach a diagnosis. Serology is the most frequently used diagnostic test for dogs.
Dogs may develop jaundice. Because it is a difficult test to maintain cdc is the only laboratory in the united states that offers the mat for leptospirosis testing for humans. Our canine leptospiral mat test code 95055 includes antibody titers against the following six leptospiral serovars. The microscopic agglutination test mat is the reference test method for the serodiagnosis of leptospirosis both in humans and in animals world health organization who 2003.
18 a 4 fold increase in titer indicates seroconversion and a diagnosis of leptospirosis. Snap lepto test pet side detection of antibodies to canine leptospira spp. Bratislava canicola grippotyphosa hardjo icterohaemorrhagiae and pomona. Test every sick dog that presents with signs consistent with leptospirosis including fever lethargy and anorexia.
Detection of antibodies using the microscopic agglutination test mat has been the most common diagnostic method used for the diagnosis of canine leptospirosis 10 vaccination with commercially available leptospirosis vaccines. 18 when paired samples are submitted the sensitivity of the mat is 100 and the specificity is 70 to 100. 19 20 a single acute. The determination of which serogroups to include depends on the geographic location of the patient.
Acute and convalescent titers may be necessary to confirm a diagnosis. Testing of 2 serum samples collected 7 to 14 days apart and representing acute and convalescent phase titers is recommended. The mat is the gold standard when testing for leptospirosis. Hence the use of the mat is preferred over the elisa.